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3 Jun 2015 Clontech Laboratories, Inc. SMARTer? RACE 5'/3' Kit User Manual Cat. No(s). 634858, 634859 (022714) Clontech Laboratories, Inc. A Takara Bio Company 1290 Terra Bella Avenue, Mountain View, CA 94043, USA U.S. Technical Support: firstname.lastname@example.org United States/Canada 800.662.2566 Asia
Should anyone else out there be familiar with RACE PCR of a different kind, attached with this email is the User Manual for the aforementioned kit, which may prove useful for comparison. Any help is much appreciated and I extend my thanks in advance for all the kind souls out there willing to do so SMARTer RACE 5'3'
SMARTer RACE 5'3' Kit User Manual_012615 - Download as PDF File (.pdf), Text File (.txt) or read online. Manual para la extraccion de ADN complementario.
provided for your convenience, but is not intended for first-time users. Generating RACE-Ready cDNA (Section V of the User Manual). Prepare enough of the following Buffer Mix for all 5'- & 3'-RACE-Ready cDNA synthesis. 1. reactions and 1 extra reaction to ensure sufficient volume. For each 10 µl cDNA synthesis reaction
Full-Length cDNA Synthesis Gets an Upgrade. It is well established that one gene codes for more than one protein. As such, it is important to have 5'- and 3'-sequence information in order to learn more about the different transcripts that result from a single gene. Full-length cDNA offers specific information about these end
3. SMARTer™ RACE cDNA Amplification Kit User Manual. I. List of Components. Cat. No. Cat. No. 634923. 634924. 10 rxns. 20 rxns. First-Strand cDNA Synthesis. 10 µl. 2x10 µl. •. SMARTer II A Oligonucleotide (12 µM). 5'–AAGCAGTGGTATCAACGCAGAGTACXXXXX–3'. (X = undisclosed base in the proprietary SMARTer
SMARTer® RACE 5'/3' Kit Protocol-At-A-Glance. (032514) www.clontech.com. Clontech Laboratories, Inc. A Takara Bio Company. Page 1 of 6. Please read the User Manual for the SMARTer® RACE 5'/3' Kit (Cat. Nos. 634858, 634859) before using this Protocol-. At-A-Glance. This abbreviated protocol is provided for your
SMARTer??????5'??????3'??cDNA???; ???10 ng?total RNA??????; ????????????????1st strand cDNA???RACE PCR; ???????????RACE cDNA?????; ???In-Fusion Kit?RACE???????????; ?PCR????SeqAmp DNA Polymerase????????????
1290 Terra Bella Avenue, Mountain View, CA 94043, USA. U.S. Technical Support: techUS@takarabio.com. United States/Canada. 800.662.2566. Asia Pacific. +1.650.919.7300. Europe. +33.(0)1.3904.6880. Japan. +81.(0)77.565.6999. Page 1 of 30. Takara Bio USA, Inc. SMARTer® RACE 5'/3'. Kit User Manual. Cat. Nos.
27 Aug 2007 SMART™ RACE cDNA Amplification Kit User Manual. Table of Contents continued. List of Figures. Figure 1. Mechanism of SMART cDNA synthesis. 4. Figure 2. Overview of the SMART RACE procedure. 6. Figure 3. The relationship of gene-specific primers to the cDNA template. 13. Figure 4. 5'- and
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